Description:
Mouse anti horse IgE, clone 3H10, recognizes native equine IgE and does not cross react with equine IgM, IgA or IgG.
IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations.
IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following infection.
Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised Balb/c mice were fused with cells of the P3X myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
ELISA, immunohistology - frozen, western blotting.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody