Mouse anti Horse IgE:7H2

Description:

Mouse anti horse IgE antibody, clone 7H2 recognizes native equine IgE and does not cross react with equine IgM, IgA or IgG.

IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 7H2 demonstrates a single major band of approximately 200 kDa under non-reducing conditions that corresponds with the expected molecular weight of the complete equine IgE molecule.

IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following worm infection.

Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Equine IgE.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised Balb/c mice were fused with cells of the P3X myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, immunohistology - frozen, immunohistology - paraffin, western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.