Mouse anti Horse Pan B-Cells:FITC

Description:

Mouse anti horse pan B-cells, clone CVS36 is a monoclonal antibody directed against equine Ig light chains. Mouse anti horse pan B-cells, clone CVS36 binds 100% of CD5^-ve peripheral blood lymphocytes and recognized all equine B-cells. As the antigen recognized by clone CVS36 appears to be present on the surface of all equine B-cells it is therefore is a reagent that can be used as a pan B-cell marker for domestic horses.

Specific anti equine reagents have yet to be fully characterized for the typically recognized B-cell makers such as CD19, CD20, CD21, CD22 and CD79. While testing has demonstrated the cross reactivity of some monoclonal B-cell markers raised against other species with equine B cells, such as the Mouse anti Human CD79a antibody, (clone HM57), an overview of which may be found in the report of the second equine leucocyte antigen workshop.

With specificity for equine Ig light chains, clone CVS36 may be used to detect all equine immunoglobulin classes and subclasses in ELISA applications.

Product Form:

Purified IgG conjugated to fluorescein isothiocyanate isomer 1 (FITC) - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.

Immunogen:

Purified Equine Ig.

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.