Mouse anti Human CD14

Description:

Mouse anti human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen. CD14 is a ~55 kDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage, a soluble form of CD14 also exists.

CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells. CD14 functions as a pattern recognition receptor in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria.

Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manne. Use of the anti-human CD14 antibody, Low Endotoxin format is recommended for this purpose.

Product Form:

Purified IgG - liquid.

Preparation:

Pack size: 0.1 mg: Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Pack size: 0.2 mg: Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Pack size: 25 µg: Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

Pack Size: 0.1 mg
0.09% Sodium azide (NaN₃).
Pack Size: 0.2 mg, 25 µg
0.09% Sodium azide.

Carrier Free:

Yes.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry, immunofluorescence, western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.