Mouse anti Human CD146

Description:

Mouse anti human CD146 antibody, clone OJ79c recognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule (MCAM) or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single pass type 1 transmembrane glycoprotein with a calculated molecular mass of ~72 kDa. However due to extensive N-linked glycosylation CD146 migrates in polyacrylamide gels with an apparent molecular mass of ~118 kDa. CD146 is a member of the immunoglobulin superfamily bearing 2 V-type Ig-like and 3 C-type Ig-like domains. CD146 is expressed by all endothelial cells and by melanoma cells and appears to act as an adhesion molecule. Expression in melanoma may be linked to tumor progression.

Mouse anti human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

Pack size: 25 µg
0.09% Sodium azide.
Pack size: 0.2 mg
0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Recombinant human MUC18 (D1-D5) Fc protein.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry, immunofluorescence, immunohistology - frozen.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.