Mouse anti Human CD14:RPE

Description:

Mouse anti human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen. CD14 is a ~55 kDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage, a soluble form of CD14 also exists.

CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells. CD14 functions as a pattern recognition receptor in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria.

Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner. Use of the anti-human CD14 antibody, low endotoxin format is recommended for this purpose.

Product Form:

Purified IgG conjugated to RPE - lyophilized.

Reconstitution:

Pack size: 100 Tests
Reconstitute with 1ml distilled water.
Pack size: 25 Tests
Reconstitute with 0.25ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.
1.0% Bovine serum albumin.
5% Sucrose.

Excitation Max (nm):

RPE 488 nm laser: 496.

Emission Max (nm):

RPE 488 nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood.

Storage:

Pack size: 100 tests
Prior to reconstitution Store at +4°C.
After reconstitution Store at +4°C.
DO NOT FREEZE.

Pack size: 25 tests
Store at +4°C. DO NOT FREEZE.

Note:

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.