Mouse anti human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen. CD14 is a ~55 kDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage, a soluble form of CD14 also exists.
CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells. CD14 functions as a pattern recognition receptor in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria.
Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner. Use of the anti-human CD14 antibody, low endotoxin format is recommended for this purpose.
Product Form:
Purified IgG conjugated to RPE - lyophilized.
Reconstitution:
Pack size: 100 tests Reconstitute with 1ml distilled water. Pack size: 25 tests Reconstitute with 0.25ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells or 100µl whole blood.
Storage:
Pack size: 100 tests Prior to reconstitution Store at +4°C. After reconstitution Store at +4°C. DO NOT FREEZE.
Pack size: 25 tests Store at +4°C. DO NOT FREEZE.
Note:
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.