Overview

Description:

Mouse anti human CD16 antibody, clone KD1 recognizes human CD16, a 50-65 kDa cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes. Clone KD1 recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16.

Mouse anti human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine and ovine.

Product Form:

Purified IgG conjugated to FITC - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.

Immunogen:

A polyclonal population of NK cells.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised mice were fused with cells of the P3U1 myeloma cell line.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Product Type:

Monoclonal antibody

Clone:

KD1

Isotype:

IgG2a

Specificity:

CD16

Species Cross-Reactivity:

Bovine, sheep, horse, pig, rat, dog, dolphin

Target:

Human

Mouse anti Human CD16:FITC

Overview

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