Overview

Description:

Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines.
Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Immunogen:

Normal human blood lymphocytes.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunized mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunoprecipitation.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Product Type:

Monoclonal antibody

Clone:

LT27

Isotype:

IgG2a

Specificity:

CD27

Species Cross-Reactivity:

Sheep, cynomolgus monkey, rhesus monkey

Target:

Human

Mouse anti Human CD27

Overview

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