Mouse anti Human CD27:FITC
Products Name
Mouse anti Human CD27:FITC
Cat.No.
SCSA0044
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Overview
Description:
Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines.
Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.
Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.
Product Type:
Monoclonal antibody
Clone:
LT27
Isotype:
IgG2a
Specificity:
CD27
Species Cross-Reactivity:
Sheep, cynomolgus monkey, rhesus monkey
Target:
Human
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by ion exchange chromatography.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
Immunogen:
Normal human blood lymphocytes.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunized mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
