Mouse anti Human CD32:Biotin

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Mouse anti Human CD32:Biotin
Products Name
Mouse anti Human CD32:Biotin
Cat.No.
CDMA0024
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Overview

Description:
Mouse anti human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.

Mouse anti human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII.
Product Form:
Purified IgG conjugated to biotin - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
Immunogen:
K562 cell line.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells or 100 µl whole blood.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody
Clone:
AT10
Isotype:
IgG1
Specificity:
CD32
Species Cross-Reactivity:
Dog, rhesus monkey, pig
Target:
Human

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