Mouse anti Human CD32:FITC
Products Name
Mouse anti Human CD32:FITC
Cat.No.
CDMA0025
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Overview
Description:
Mouse anti human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.
Mouse anti human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII.
Mouse anti human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII.
Product Type:
Monoclonal antibody
Clone:
AT10
Isotype:
IgG1
Specificity:
CD32
Species Cross-Reactivity:
Dog, rhesus monkey, pig
Target:
Human
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
1% Bovine serum albumin.
Immunogen:
K562 cell line.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells or 100 µl whole blood.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
