Overview

Description:

Mouse anti human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.

Mouse anti human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII.

Product Form:

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.

Reconstitution:

Pack size: 100 tests
Reconstitute with 1.0 ml distilled water.
Pack size: 25 tests
Reconstitute with 0.25ml distilled water.

Preparation:

Purified IgG prepared by affinity chromatography on protein A.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

Pack size: 100 tests
0.09% Sodium azide.
1% Bovine serum albumin.
Pack size: 25 tests
0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.
5% Sucrose.

Immunogen:

K562 cell line.

Fusion Partners:

Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Excitation Max (nm):

RPE 488 nm laser: 496.

Emission Max (nm):

RPE 488 nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells or 100 µl whole blood.

Storage:

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.

Note:

DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.

Product Type:

Monoclonal antibody

Clone:

AT10

Isotype:

IgG1

Specificity:

CD32

Species Cross-Reactivity:

Dog, rhesus monkey, pig

Target:

Human

Mouse anti Human CD32:RPE

Overview

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