Mouse anti Human CD49d

Description:

Mouse anti human CD49d monoclonal antibody, clone HP2/1 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa. CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form the Peyer's patch-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue through its interaction with MadCam-1, preferentially expressed on Peyer’s patch high endothelial venules and postcapillary venules in lamina propria.
Mouse anti human CD49d, clone HP2/1 binds to both intact and the 80kDa fragment of integrin alpha-4. CD49d is expressed on monocytes, T cells, B cells, thymocytes and Langerhans cells.
Mouse anti human CD49d, clone HP2/1 can be used in assays of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and inhibits cell binding to soluble VCAM-1.

Product Form:

Purified IgG - liquid.

Preparation:

Pack size: 0.2 mg
Purified IgG prepared from tissue culture supernatant.
Pack size: 0.1 mg
Purified IgG prepared by affinity chromatography from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

Pack size: 0.2 mg
0.1% Sodium azide.
Pack size: 0.1 mg
<0.1% Sodium azide (NaN₃).

Immunogen:

JM leukaemia line.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunized BALB/c mice were fused with cells of the X63 Ag8.653 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, functional assays, immunohistology - frozen, immunoprecipitation.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.