Description:
Mouse anti human CD62P antibody, clone Psel.KO.2.5 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein, also known as P-selectin.
CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
Product Form:
Purified IgG conjugated to RPE - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
CD62P transfected 300.19 cells.
Fusion Partners:
Spleen cells from immunised CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody
Species Cross-Reactivity:
Pig, sheep