Description:
Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. Mouse anti human CD88 antibody, clone S5/1 recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21).
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
Immunogen:
C5aR - peptide: Met1 - Asn31.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Product Type:
Monoclonal antibody
Species Cross-Reactivity:
Rabbit, bovine, ferret, mink