Mouse anti Human Cytochrome C

Description:

Mouse anti human cytochrome C antibody, clone 2G8 recognises cytochrome c, originally termed Apaf-2, an evolutionarily conserved protein which acts as an essential mitochondrial electron carrier between respirable substrates and oxygen, during aerobic energy production.

A second major function for cytochrome c is its role as an intermediate in apoptosis, both through its association with inositol-3-phosphate receptor (IP3 receptor) which acts as an ER calcium release channel, and its association with apoptotic protease activating factor 1 (Apaf-1), resulting in the formation of the apoptosome protein complex which then recruits and activates pro-caspase 9 (Apaf-3).

Mouse anti human cytochrome C antibody, clone 2G8 is reported to block the role of cytochrome c as a co-factor in the activation of caspase 9 . Removal of sodium azide is recommended prior to use in functional assays.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

< 0.1% Sodium azide (NaN₃).

Immunogen:

Human cytochrome c.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry, western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.