Mouse anti Human Macrophages:Low Endotoxin

Description:

Mouse anti human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains subsequent evidence indicates that the antibody detects an epitope exclusively expressed on the beta chain demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition, Mouse anti human macrophages, clone MAC387 detects the beta chain in complex with the alpha.

The antigen recognized by Mouse anti human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

None present.

Endotoxin Level:

<0.01EU/µg

Carrier Free:

Yes

Immunogen:

Human monocytes.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunohistology - paraffin.

Storage:

Store at -20°C only.
This product should be stored undiluted.

Note:

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.