Mouse anti Mouse MHC Class I:FITC
Products Name
Mouse anti Mouse MHC Class I:FITC
Cat.No.
PCMA0122
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Overview
Description:
Mouse anti mouse MHC Class I antibody, clone 2G5 recognizes a monomorphic epitope present on murine MHC class I molecules, expressed at varying levels on the majority of nucleated cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
The epitope recognized by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this antibody may recognize a polymorphic epitope of canine MHC class I.
The epitope recognized by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this antibody may recognize a polymorphic epitope of canine MHC class I.
Product Type:
Monoclonal antibody
Clone:
2G5
Isotype:
IgG2b
Specificity:
MHC Class I
Species Cross-Reactivity:
Pig, rat, bovine, sheep
Target:
Mouse
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
1% Bovine serum albumin.
Immunogen:
Purified H-2Kb and H-2Db MHC-I molecules.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
