Mouse anti PCNA:HRP

Description:

Mouse anti PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork through its interaction with FEN1. PCNA is the auxilliary protein for DNA polymerase δ.

PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse, chicken and abalone.

Product Form:

Purified IgG conjugated to HRP - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.01% Thiomersal.
HRP stabiliser.

Immunogen:

Rat PCNA made in the protein A expression vector pR1T2T.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Western blotting.

Storage:

Store at +4°C.

Note:

DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.