Mouse anti Pig CD11R3

Description:

Mouse anti pig CD11R3, clone 2F4/11 recognizes porcine CD11R3, a ~155 kDa cell surface glycoprotein, member of the alpha integrin family.
Mouse anti pig CD11R3, clone 2F4/11 was clustered as CD11R3 at the Third International Workshop on swine leukocyte differentiation antigens. CD11R3 has a similar expression pattern to the human CD11b marker, being expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets.

Mouse anti pig CD11R3, clone 2F4/11 is reported to block phagocytosis of complement-opsonized zymosan particles by polymorphonuclear granulocytes and alveolar macrophages.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Porcine alveolar macrophages.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunoprecipitation, western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.