Mouse anti Pig CD11R3:FITC

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Mouse anti Pig CD11R3:FITC
Products Name
Mouse anti Pig CD11R3:FITC
Cat.No.
PCMA0011
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Overview

Description:
Mouse anti pig CD11R3, clone 2F4/11 recognizes porcine CD11R3, a ~155 kDa cell surface glycoprotein, member of the alpha integrin family.
Mouse anti pig CD11R3, clone 2F4/11 was clustered as CD11R3 at the Third International Workshop on swine leukocyte differentiation antigens. CD11R3 has a similar expression pattern to the human CD11b marker, being expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets.

Mouse anti pig CD11R3, clone 2F4/11 is reported to block phagocytosis of complement-opsonized zymosan particles by polymorphonuclear granulocytes and alveolar macrophages.
Product Type:
Monoclonal antibody
Clone:
2F4/11
Isotype:
IgG1
Specificity:
CD11R3
Target:
Pig
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
Immunogen:
Porcine alveolar macrophages.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

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