Description:
Mouse anti pig CD11R3, clone 2F4/11 recognizes porcine CD11R3, a ~155 kDa cell surface glycoprotein, member of the alpha integrin family.
Mouse anti pig CD11R3, clone 2F4/11 was clustered as CD11R3 at the Third International Workshop on swine leukocyte differentiation antigens. CD11R3 has a similar expression pattern to the human CD11b marker, being expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets.
Mouse anti pig CD11R3, clone 2F4/11 is reported to block phagocytosis of complement-opsonized zymosan particles by polymorphonuclear granulocytes and alveolar macrophages.
Product Form:
Purified IgG conjugated to RPE - liquid.
Reconstitution:
Reconstitute with 1 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
Porcine alveolar macrophages.
Fusion Partners:
Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
RPE 561nm laser: 546.
Emission Max (nm):
RPE 488 nm laser: 578.
RPE 561nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Store at +4°C DO NOT FREEZE.
Note:
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody