Mouse anti Pig CD14:FITC

Description:

Mouse anti pig CD14, clone MIL2 recognizes porcine CD14. Clone MIL2 was clustered as porcine CD14 at the Third International Workshop on swine leukocyte differentiation antigens. Mouse anti pig CD14, clone MIL2 immunoprecipitates a protein of ~50 kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition assays. Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site.

Mouse anti pig CD14, clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry with a similar expression pattern to the anti human CD14 clone TüK4, lymphocytes and eosinophils are negative for MIL2 staining. Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells.

Product Form:

Purified IgG conjugated to FITC - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.

Immunogen:

Porcine peripheral blood lymphocytes.

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from immunized Balb/c mice were fused with cells from the P2-X63-Ag.653 mouse myeloma.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.