Mouse anti Pig CD205

Description:

Mouse anti pig CD205, clone ZH9F7 recognizes the endocytic receptor CD205, also known as DEC205.
CD205 is expressed at high levels by dendritic cell (DC) subsets and can be detected on thymic epithelial cells.
Mouse anti pig CD205 antibody, clone ZH9F7, has been used in characterization of the species-conserved features of the cDC1 subset. This subset is characterised by high surface expression of CD205, CD135, CADM1, low levels of CD172a, a lack of CD115, XCR1, and BATF3; and restricted APN gene expression. CD205 receptor expression was confirmed in cDC1 and cDC2 subsets using flow cytometry on porcine tonsil, submaxillary and mesenteric lymph nodes, and spleen lymphoid tissues.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Pig CD205.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.