Mouse anti Pig CD27:APC

Description:

Mouse anti pig CD27 antibody, clone B30C7 recognizes the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family. In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however, using the B30C7 clone, expression of CD27 on porcine B-cells appears undetectable.
Porcine CD27 is expressed by all naïve CD8a^-ve T-helper cells and a sub-population of CD8a^+ve cells.
Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and clone B30C7 may be used to differentiate between subsets of pig NK cells.

Product Form:

Purified IgG conjugated to Allophycocyanin (APC) - lyophilized.

Reconstitution:

Reconstitute with 1.0 ml distilled water.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.
5% Sucrose.

Immunogen:

Porcine peripheral blood monocytes.

Fusion Partners:

Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line.

Excitation Max (nm):

APC: 650.

Emission Max (nm):

APC: 661.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.

Note:

DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.