Mouse anti Pig CD31:RPE

Description:

Mouse anti pig CD31, clone LCI-4 recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types. The extracellular region contains six Ig-like domains. Mouse anti pig CD31, clone LCI-4 is cross reactive with human CD31 and binds to the 5^th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutants.
Mouse anti pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions.

Product Form:

Purified IgG conjugated to RPE - liquid.

Reconstitution:

Pack size: 100 tests
Reconstitute with 1 ml distilled water.
Pack size: 25 tests
Reconstitute with 0.25 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.

Preparation:

Purified IgG prepared by affinity chromatography from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.
5% Sucrose.

Immunogen:

Porcine CD31/human IgGFc fusion protein.

Excitation Max (nm):

RPE 488 nm laser: 496.

Emission Max (nm):

RPE 488 nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells or 100 µl whole blood.

Storage:

Store at +4°C.

Note:

DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.