Mouse anti Pig CD335:APC

Description:

Mouse anti pig CD335 antibody, clone VIV-KM1 recognizes the porcine homologue of human CD335, also known as NKp46 and natural cytotoxicity triggering receptor 1 (NCR1), a member of the natural cytotoxicity receptor (NCR) family.

CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor and is involved in the control of viral infection and tumor development. CD335 is expressed by human natural killer cells. The development of monoclonal antibodies to bovine CD335 (clone AKS1) and ovine CD335 (clone EC1.1) have enabled researchers to identify and better understand ruminant NK cells.

Mouse anti pig CD335 antibody, clone VIV-KM is the first monoclonal developed to specifically identify porcine CD335 and provides a reagent to facilitate a better understanding of the pig immune system and aid in the understanding of the role of NK cells in host pathogen defense. Porcine CD335 is not expressed by all NK cells and expression may be influenced by cytokine production.

Product Form:

Purified IgG conjugated to Allophycocyanin (APC) - lyophilized.

Reconstitution:

Reconstitute with 1.0 ml distilled water.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.
5% Sucrose.

Immunogen:

Fusion protein consisting of the extracellular region of porcine CD335.

Fusion Partners:

Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.

Excitation Max (nm):

APC: 650.

Emission Max (nm):

APC: 661.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.

Note:

DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.