Mouse anti Pig CD45 Allotypic Variant

Description:

Mouse anti pig CD45 allotypic variant antibody, clone MAC323 recognizes an allotypic determinant of porcine CD45, also known as the leucocyte common antigen (LCA). Mouse anti pig CD45 allotypic variant antibody, clone MAC323 has been shown to recognize CD45 in pigs of the Large White and German Landrace strains. Another antibody, clone K252.1E4, recognizes a monomorphic determinant on porcine CD45. When K252.1E4 is used in conjunction with MAC323, it can be shown that MAC323 stains a subset of the leukocytes that are detected by K252.1E4; indicating a loss of the epitope in MAC323^-ve animals rather than loss of the CD45 antigen. Cross-breeding positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelian autosomal dominant pattern.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Porcine peripheral blood lymphocytes.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunohistology - paraffin, immunohistology - resin, immunoprecipitation.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.