Mouse anti Pig CD45RC:RPE

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Mouse anti Pig CD45RC:RPE
Products Name
Mouse anti Pig CD45RC:RPE
Cat.No.
PCMA0080
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Overview

Description:
Mouse anti pig CD45RC, clone 3a56 recognizes a restricted CD45 epitope encoded by the CD45 C exon and will detect the ~210 kDa RC isoform.
CD45 in pigs has three cell surface expressed isoforms: CD45R, CD45RA, and CD45RC. CD45RC is mainly expressed by CD4+ T cells and weakly by B cells. However, during the immune response expression of CD45RC was found to decrease in activated T-helper cells. In contrast, increased expression of CD45RC was observed during the maturation of CD4-  gamma delta thymocytes.
Product Form:
Purified IgG conjugated to RPE - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
5% Sucrose.
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Excitation Max (nm):
RPE 488 nm laser: 496.
RPE 561 nm laser: 546.
Emission Max (nm):
RPE 488 nm laser: 578.
RPE 561 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Store at +4°C.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Product Type:
Monoclonal antibody
Clone:
3a56
Isotype:
IgG1
Specificity:
CD45RC
Target:
Pig

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