Mouse anti Pig CD61

Description:

Mouse anti pig CD61 antibody, clone JM2E5 recognizes the porcine CD61 cell surface antigen, also known as platelet glycoprotein IIIa or integrin beta.
CD61 is present on the megakaryocyte/platelet lineage, granulocytes, cells from the monocyte/macrophage lineage and endothelial cells. CD61 is also broadly expressed on tissues, such as epithelial cells from tubules in the kidney, spleen, intestinal mucosa and Leydig cells in testis.
Mouse anti pig CD61 antibody, clone JM2E5 detects a band of approximately 85 kDa in porcine platelet lysates by western blotting. The epitope recognized by this antibody is not sensitive to EDTA.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Immunogen:

Porcine peripheral blood mononuclear cells.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunohistology - paraffin, immunoprecipitation, Western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.