Description:
Mouse anti pig CD8 alpha antibody, clone 11/295/33 recognizes an epitope on the alpha chain of porcine CD8. Clone 11/295/33 detects a different epitope from that bound by clone 76-2-11.
In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver. Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells.
Product Type:
Monoclonal antibody
Product Form:
Purified IgG conjugated to RPE - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
Porcine lymphocytes.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.