Mouse anti Pig Granulocytes/6D10:FITC

Description:

Mouse anti pig Granulocytes antibody, clone 6D10 recognizes a ~60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.

Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2, allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages:6D10^-2B2^- early myeloid precursors, 6D10⁺2B2^- immature neutrophils, 6D10⁺2B2⁺ mature neutrophils and 6D10^-2B2⁺ mature eosinophils and basophils.

Mouse anti pig Granulocytes antibody, clone 6D10 has been shown as suitable for use on cytospins.

Product Type:

Monoclonal antibody

Clone:

6D10

Isotype:

IgG2a

Specificity:

Granulocytes

Target:

Pig

Product Form:

Purified IgG conjugated to FITC - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.

Immunogen:

Porcine bone marrow hematopoietic cells (BMHC).

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 mouse myeloma cell line.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.