Mouse anti Pig Granulocytes:FITC

Description:

Mouse anti pig granulocytes antibody, clone 2B2 recognizes porcine basophils, neutrophils and eosinophils, acting as a reliable tool for their analysis and isolation, without contamination from other cells.

Analysis of porcine bone marrow and blood shows that the antigen recognized by clone 2B2 is selectively expressed on mature cells and that it is likely to be a labile conformational epitope. Use of clone 2B2 in conjunction with clone 6D10 product code, allows for the discrimination and characterization of different porcine granulocyte lineages and also their developmental stages: 6D10^-2B2^- early myeloid precursors, 6D10⁺2B2^- immature neutrophils, 6D10⁺2B2⁺mature neutrophils and 6D10^-2B2⁺ mature eosinophils and basophils. Clone 2B2 has been shown as suitable for use on cytospins.

Product Type:

Monoclonal antibody

Clone:

2B2

Isotype:

IgG1

Specificity:

Granulocytes

Target:

Pig

Product Form:

Purified IgG conjugated to FITC - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.

Immunogen:

Porcine bone marrow hematopoietic cells (BMHC).

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 mouse myeloma cell line.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.