Description:
Mouse anti pig IgG1, clone K139 3C8 recognizes porcine IgG1 and no cross-reaction has been observed with porcine IgA, IgG2 or IgM.
IgG1, along with IgG2 (IgG2a and IgG2b), IgG3 and IgG4 comprise the major known subclasses of IgG in swine. Combined, the various subclasses of IgG comprise approximately 85% of immunoglobulin in porcine serum.
In addition to clone K139 3C8, a range of monoclonal antibodies recognizing other porcine IgG subclasses and immunoglobulins are available.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
ELISA, immunohistology - frozen, immunohistology - paraffin, immunohistology - resin.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody