Mouse anti Pig SLA Class II DR:FITC

Description:

Mouse anti pig SLA Class II DR antibody, clone 2E9/13 recognizes SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Mouse anti pig SLA Class II DR antibody, clone 289/13 reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognizes a monomorphic determinant of porcine SLA DR.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.

Mouse anti pig SLA class II DR, clone 2E9/13 immunoprecipitates a heterodimer composed of two polypeptides of ~28 and ~35 kDa from NP-40 extracts of biotin surface-labeled porcine peripheral blood mononuclear cells. Mouse anti pig SLA Class II DR antibody, clone 289/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B.

Product Type:

Monoclonal antibody

Clone:

2E9/13

Isotype:

IgG2b

Specificity:

SLA Class II DR

Species Cross-Reactivity:

Bovine

Target:

Pig

Product Form:

Purified IgG conjugated to FITC - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.
1% Bovine serum albumin.

Immunogen:

Porcine monocytes.

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.