Description:
Mouse anti pig SWC8, clone MIL3, recognizes the porcine SWC8 cell surface antigen, an antigen that as yet has no identified human homolog. SWC8 is expressed by granulocytes, B cells, a subset of T cells and by some non-haematopoietic cells. Monocytes however do not express SWC8.
Clone MIL3 has been used in two colour flow cytometry with Mouse anti Porcine CD14 antibody, clone MIL2 to distinguish between monocytes and granulocytes.
Product Type:
Monoclonal antibody
Product Form:
Tissue culture supernatant - liquid.
Preparation:
Tissue culture supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
Immunogen:
Porcine lamina propria leucocytes.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag.653 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.