Mouse anti Pig wCD6

Description:

Mouse anti pig wCD6, clone MIL8 reacts with pig wCD6, the porcine homolog of human CD6.
Clone MIL8 was clustered as wCD6 at the Second International Swine CD Antigen Workshop based on its staining pattern of various cell types which was in complete concordance with those exhibited by the reference antibody for the wCD6 cluster at the second workshop, a38b2. Clone MIL8 and clone a38b2 immunoprecipitated a monomeric protein of ~150 kDa from an activated T-cell lysate. Further, it was established that clones MIL8 and a38b2 bind to different epitopes on porcine wCD6 due to their total lack of reciprocal binding to wCD6 expressing cells.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Porcine Lamina propria leucocytes.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised BALB/c mice were fused with cells of the P3X63 Ag.653 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.