Mouse anti Pig wCD8 Alpha:RPE
Products Name
Mouse anti Pig wCD8 Alpha:RPE
Cat.No.
PCMA0110
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Overview
Description:
Mouse anti pig wCD8 alpha antibody, clone MIL12 recognizes an epitope on the alpha chain of porcine wCD8. Clone MIl12 was clustered at the Third International Swine CD Workshop. Mouse anti pig wCD8 alpha antibody, clone MIL12 was determined to bind to the CD8a epitope on the alpha chain based on its staining pattern on T lymphocytes and on its ability to block binding of the previously characterized CD8a antibody clone 76-2-11 to T lymphocytes.
Product Type:
Monoclonal antibody
Clone:
MIL12
Isotype:
IgG2a
Specificity:
CD8 alpha
Target:
Pig
Product Form:
Purified IgG conjugated to RPE - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
Porcine mesenteric lymphocytes.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
