Description:
Mouse anti rabbit CD45 antibody, clone L12/201 recognizes the CD45 antigen, also known as leukocyte common antigen (LCA) or T200. Mouse anti rabbit CD45 antibody, clone L12/201 shows pan leucocyte reactivity by flow cytometry and immunohistochemistry.
Immunoprecipitation was achieved by cross linking antibody to the labelled cell surface yielding a protein migrating by gel electrophoresis at a molecular mass of ~200 kDa.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Immunogen:
Glycoproteins isolated from the T cell line, RL-5.
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry, immunohistology - frozen, immunofluorescence.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody