Mouse anti rabbit CD45 antibody, clone L12/201 recognizes the CD45 antigen, also known as leukocyte common antigen (LCA) or T200. Mouse anti rabbit CD45 antibody, clone L12/201 shows pan leucocyte reactivity by flow cytometry and immunohistochemistry.
Immunoprecipitation was achieved by cross linking antibody to the labelled cell surface yielding a protein migrating by gel electrophoresis at a molecular mass of ~200 kDa.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Carrier Free:
Yes.
Immunogen:
Glycoproteins isolated from the T cell line, RL-5.
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody
Clone:
L12/201
Isotype:
IgG1
Specificity:
CD45
Target:
Rabbit
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