Mouse anti Rabbit IgM (B Cell Marker)
Products Name
Mouse anti Rabbit IgM (B Cell Marker)
Cat.No.
RCMA0037
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Overview
Description:
Mouse anti rabbit IgM antibody, clone NRBM labels IgM+ve B cells (Dewals et al. 2011, Waclavicek et al. 2009) and as such can be considered a reliable marker of lagomorph B cells for flow cytometry.
Mammalian IgM is produced and secreted by plasma cells located in bone marrow, lymph nodes and spleen. IgM is present in both a secreted polymeric form and as cell surface monomeric form on B cells.
Mammalian IgM is produced and secreted by plasma cells located in bone marrow, lymph nodes and spleen. IgM is present in both a secreted polymeric form and as cell surface monomeric form on B cells.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised mice were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry, immunohistology - frozen.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody
Clone:
NRBM
Isotype:
IgG1
Specificity:
IgM
Region:
(B-Cell Marker)
Target:
Rabbit
