Overview

Description:

Mouse anti rabbit IgM (B cell marker) antibody, clone NRBM recognizes rabbit IgM.

Mammalian IgM is produced and secreted by plasma cells located in bone marrow, lymph nodes and spleen. IgM is present in both a secreted polymeric form and as cell surface monomeric form on B cells.

Mouse anti rabbit IgM antibody, clone NRBM labels IgM^+ve B cells and as such can be considered a reliable marker of lagomorph B cells for flow cytometry.

Product Form:

Purified IgG conjugated to FITC - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from immunised mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Product Type:

Monoclonal antibody

Clone:

NRBM

Isotype:

IgG1

Specificity:

IgM

Region:

B-cell marker

Target:

Rabbit

Mouse anti Rabbit IgM (B Cell Marker):FITC

Overview

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