Mouse anti Rabbit IgM (B Cell Marker):RPE
Products Name
Mouse anti Rabbit IgM (B Cell Marker):RPE
Cat.No.
RRIA0032
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Overview
Description:
Mouse anti rabbit IgM (B cell marker) antibody, clone NRBM recognizes rabbit IgM.
Mammalian IgM is produced and secreted by plasma cells located in bone marrow, lymph nodes and spleen. IgM is present in both a secreted polymeric form and as cell surface monomeric form on B cells.
Mouse anti rabbit IgM antibody, clone NRBM labels IgM+ve B cells and as such can be considered a reliable marker of lagomorph B cells for flow cytometry.
Mammalian IgM is produced and secreted by plasma cells located in bone marrow, lymph nodes and spleen. IgM is present in both a secreted polymeric form and as cell surface monomeric form on B cells.
Mouse anti rabbit IgM antibody, clone NRBM labels IgM+ve B cells and as such can be considered a reliable marker of lagomorph B cells for flow cytometry.
Product Form:
Purified IgG conjugated to RPE - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
5% Sucrose.
1% Bovine serum albumin.
5% Sucrose.
Fusion Partners:
Spleen cells from immunised mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Product Type:
Monoclonal antibody
Clone:
NRBM
Isotype:
IgG1
Specificity:
IgM
Region:
B-cell marker
Target:
Rabbit
