Mouse anti Rabbit MHC Class II DQ

Description:

Mouse anti rabbit MHC class II recognizes the RLA DQ molecule, expressed by antigen presenting cells, B cells and monocytes. Mouse anti rabbit MHC class II monoclonal antibody, clone 2C4 recognizes the class II RLA-DQ molecule. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rabbits, this complex is referred to as the rabbit leukocyte antigen (RLA) region. There are 3 major MHC class II proteins encoded by the RLA which are RLA DP, RLA DQ and RLA DR.

Mouse anti rabbit MHC class II monoclonal antibody, clone 2C4 is able to significantly inhibit the mixed lymphocyte reaction. Immunoprecipitation with the antibody yields three bands of molecular weights 28, 31 and 35 kDa.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Rabbit spleen cells.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised mice were fused with cells of the P3.X63. Ag8.653 mouse myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunoprecipitation.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.