Description:
The monoclonal antibody 2C4 reacts with RLA-DQ-transfected cells, but not with RLA-DR-transfected cells. In normal adult rabbits, the antibody reacts with 30-60% of peripheral blood leukocytes. It has also been reported that the antibody stains lymphocytes and macrophages in the spleen, lymphocytes in the thymic medulla and some cells in the cortex, Kupffer cells in the liver, and synovial lining cells (e.g., infiltrating macrophages) in inflamed synovium. DQα transcripts have been detected in bone marrow and spleen and to a lesser extent in appendix, lymph nodes, and other organs.
Immunogen:
Rabbit spleen cells
Alternative Names:
MHC Class II, leukocyte antigen
Conjugate:
PE (R-phycoerythrin)
Buffer :
Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Recommended Storage:
2-8°C, Avoid exposure to light, Do not freeze
Application:
Flow cytometry
Immunohistochemistry-frozen sections
Immunohistochemistry-paraffin sections
Immunohistochemistry-while mount
Immunocytochemistry
ELISA
Western blot
Immunoprecipitation
Blocking
Isotype:
Mouse (BALB/c) IgG2aκ
Specificity:
Rabbit RLA-DQ