Mouse anti Rabbit T Lymphocytes

Description:

Mouse anti rabbit T lymphocytes, clone KEN-5, recognizes rabbit (lapine) T-cells. Clone KEN-5 recognizes >90% thymocytes and approximately 40-45% mesenteric lymph node cells and immunoprecipitates a ~67 kDa protein from rabbit thymocytes.

In contrast to humans and mice where only a very small population of B-cells express the CD5 antigen, in rabbits it is widely expressed by peripheral blood B cells. However, the KEN-5 antibody, while originally reported as recognizing rabbit CD5 (Kotani et al.1993), does not bind to rabbit CD5 transfectants. Furthermore, clone KEN-5, unlike other known antibodies recognizing rabbit CD5 and anti human cross reactive CD5 antibodies, shows very limited binding to rabbit B-cells, which in adult rabbits express CD5 (Raman & Knight 1992) Clone KEN-5 may recognize a epitope that is dependant on glycosylation to maintain its structural configuration which may explain why this antibody does not recognize recombinant rabbit CD5 produced in insect cells which contain different glycans from those found in mammalian cells. KEN-5 may distinguish between different isoforms of CD5 in lapine T and B cells (Pospisil et al. 2009).

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Rabbit thymocytes.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised BALB/c mice were fused with cells of the PAI mouse myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry, immunohistology - frozen, immunoprecipitation.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.