Mouse anti Sheep CD25:FITC
Products Name
Mouse anti Sheep CD25:FITC
Cat.No.
SCSA0041
Size
Qty
Add to Cart
Inquiry
Overview
Description:
Mouse anti sheep CD25, clone 9.14 immunoprecipitates a band of ~47 kDa under reducing conditions, as expected for the mature protein due to high glycosylation, consistent with the observed molecular weight of IL-2Rα in other species including humans.
Antibodies to CD4, FoxP3 and CD25 may be used elucidate properties of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity.
Antibodies to CD4, FoxP3 and CD25 may be used elucidate properties of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity.
Product Type:
Monoclonal antibody
Clone:
9.14
Isotype:
IgG1
Specificity:
CD25
Target:
Sheep
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
1% Bovine serum albumin.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
