Mouse anti Sheep CD335
Products Name
Mouse anti Sheep CD335
Cat.No.
MACD0026
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Overview
Description:
Mouse anti sheep CD335, clone EC1.1, recognizes the ovine homologue of human CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1.
CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which function as an activating receptor.
Research has indicated that, in mammals, the CD335 antigen is confined to NK cells and that it is primarily expressed by a subset of natural killer (NK) cells which are CD3-ve, CD4-ve and CD2+ve with a small population also being CD8+ve. No expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.
NK cells are cytotoxic and form a key component of the innate immune system where they are involved in mediating immunity to tumours and pathogens and in particular are critical to the process where the host recognizes and rejects tumour and viral infected cells.
Clone EC1.1 (originally named as Gr13.1) is the first antibody specifically raised to ovine CD335 and provides a reagent enabling researchers to identify ovine NKp46 positive NK cells and may facilitate an increased understanding of the ovine immune system and in particular its role in host pathogen defence.
In addition to clone EC1.1, clones AKS1, which recognises CD335 (NKp46) in bovine and other ruminants, and VIV-KM1, which recognizes porcine CD335, are also available from CD BioSciences.
CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which function as an activating receptor.
Research has indicated that, in mammals, the CD335 antigen is confined to NK cells and that it is primarily expressed by a subset of natural killer (NK) cells which are CD3-ve, CD4-ve and CD2+ve with a small population also being CD8+ve. No expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.
NK cells are cytotoxic and form a key component of the innate immune system where they are involved in mediating immunity to tumours and pathogens and in particular are critical to the process where the host recognizes and rejects tumour and viral infected cells.
Clone EC1.1 (originally named as Gr13.1) is the first antibody specifically raised to ovine CD335 and provides a reagent enabling researchers to identify ovine NKp46 positive NK cells and may facilitate an increased understanding of the ovine immune system and in particular its role in host pathogen defence.
In addition to clone EC1.1, clones AKS1, which recognises CD335 (NKp46) in bovine and other ruminants, and VIV-KM1, which recognizes porcine CD335, are also available from CD BioSciences.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Carrier Free:
Yes.
Immunogen:
OvNKp46-mFcγ2b fusion protein.
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised Balb/c mice were fused with cells of the X63 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry, immunohistology - frozen, immunohistology - paraffin.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody
Clone:
EC1.1
Isotype:
IgG1
Specificity:
CD335
Species Cross-Reactivity:
Goat, bovine
Target:
Sheep
