Mouse anti Sheep CD45:RPE
Products Name
Mouse anti Sheep CD45:RPE
Cat.No.
MACD0037
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Overview
Description:
Mouse anti sheep CD45 antibody, clone 1.11.32 recognizes the ovine CD45 (Leucocyte common antigen), expressed on all ovine lymphocytes, macrophages and granulocytes. Mouse anti sheep CD45 antibody, clone 1.11.32 immunoprecipitates CD45 molecules of 190 kDa, 210 kDa and 225 kDa from lymph node lysates.
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.
Reconstitution:
Reconstitute with 1 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
Ovine efferent lymphatic duct lymphocytes.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
After reconstitution store at +4°C.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody
Clone:
1.11.32
Isotype:
IgG1
Specificity:
CD45
Species Cross-Reactivity:
Goat, bovine
Target:
Sheep
