Mouse anti Sheep CD45R:RPE
Products Name
Mouse anti Sheep CD45R:RPE
Cat.No.
SCSA0072
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Overview
Description:
Mouse anti sheep CD45R antibody, clone 20.96 recognizes the ovine CD45R cell surface antigen, a ~220 kDa glycoprotein, expressed by all B lymphocytes and a subset of T lymphocytes. In immunohistochemical staining the antibody labels all lymphocytes within the B cell follicles as well as scattered cells in interfollicular areas.
Product Form:
Purified IgG conjugated to RPE - lyophilized.
Reconstitution:
Reconstitute with 1 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
Sheep thymocytes.
Fusion Partners:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
After reconstitution store at +4°C.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody
Clone:
20.96
Isotype:
IgG1
Specificity:
CD45R
Target:
Sheep
